Vogel Johnson Agar For Selective Isolation of Staphylococcus Aureus, Final pH: 7.2±0.2, 500g/bottle
- Brand:Huankai Microbial
- Model:MO00630140
- Availability:In Stock
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Vogel Johnson Agar
Usages:
For selective isolation of staphylococcus aureus.
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter):
Pancreatic Digest of Casein | 10g |
Yeast Extract | 5 g |
Mannitol | 10 g |
Dibasic Potassium Phosphate | 5 g |
Lithium chloride | 5 g |
Glycine | 10 g |
Agar | 16 g |
Phenol Red | 25mg |
Final pH | 7.2±0.2 |
How to use:
1.Suspend 61g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item | The name and number of strain | Growth | Colony Color |
1 | Staphylococcus aureus CMCC (B) 26003 | Good | Golden yellow |
2 | Staphylococcus epidermidis CMCC (B) 26069 | Good | Red |
3 | Escherichia coli CMCC (B) 44102 | Inhibition | -- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle