VRB-MUG Agar , 100g

Description: 

VRBA with MUG is a Standard Methods medium recommended by FDA/BAM¹. 

The medium is based on Violet Red Bile Lactose Agar (VRBLA) (CM0107) which is a selective medium for the detection and enumeration of coliform organisms. The medium has been used for the determination of the coli -aerogenes content of water, milk and other dairy products, dairy equipment, and food products etc. 

Organisms which rapidly attack lactose produce purple colonies surrounded by purple haloes. Non -lactose or late-lactose fermenters produce pale colonies with greenish zones. Other related Gr am-negative bacteria may grow but can be suppressed by incubation at >42°C or by anaerobic incubation. 

Druce et al. found that Violet Red Bile Lactose Agar was as good an indicator of coli -aerogenes bacteria in milk as MacConkey Broth, and that the Oxoid medium was suitable for determining the coli -aerogenes content of milk. 

VRBA with MUG contains 4-methylumbelliferyl-ß-D-glucuronide (MUG) as a substrate which is cleaved by the enzyme ß-glucuronidase produced by Escherichia coli resulting in a fluorescent e nd-product. 

NaOH addition generally improves fluorescence by increasing the alkalinity of the solution. An increase in fluorescence can also be achieved by increasing the level of MUG added to the base medium. 

Specification: 

100g