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Description:
The composition of this medium is in accordance with the harmonized methodology of USP/EP/BP/JP/IP (1,2,3,4,5). This medium is recommended for preparation of stable test strain suspension employed for validating the microbiological testing procedures of non-sterile products. The standardized stable suspensions are used so that the suitability of this test to detect microorganism in presence of p roduct can be established. Non-fatty products insoluble in water and water-soluble products are diluted/dissolved using this solution.
HMC Peptone serves as nutrient source and maintains the cell viability. Phosphates in the medium act as good buffering ag ents. Sodium chloride maintains the osmotic balance and cell integrity. Polysorbates reduce surface tension and also inactivate phenolic compound, if present in the test sample. Preparation of test strain is recommended in Buffered Sodium chloride-Peptone solution pH 7.0 (MH1275) at 30-35°C wherein there is no multiplication of organisms or there is no decrease in count for upto 4 hours.
Specification:
250g