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Description:
Bile Salt Agar is formulated as recommended by BIS (1) for isolation, identification and enumeration of Vibrio cholerae . Vibrio species, like many other gram-negative bacteria, grow in the presence of relatively high levels of bile salts (2). On Bile Salt Agar colonies of Vibrios have a distinctive appearance which may be seen by growing a known strain of Vibrio cholerae and comparing it with Escherichia coli (1).
Suspicious growths may be tested by slide agglutination using polyvalent cholera typing serum. The medium contains peptic digest of animal tissue, m eat extract which provides carbonaceous, nitrogenous compounds and other essential growth nutrients. Sodium chloride buffers the medium well. Sodium taurocholate inhibits most of the gramnegative organisms.
Specification:
250g