Manganese Nutrition Agar , 250g

Description: 

Nutrient Agar w/ Manganese, conventionally abbreviated as NAMn favours culture and sporulation of aerobic Bacillus species especially from canned foods. Beef extract and pancreatic digest of gelatin provide necessary nutrients for growth of Bacillus species. Manganese is known to influence and enhance sporulation in Bacillus species (1- 4). It has been reported that organisms recovered from spoilage of foods such as fruit drinks, tomatoes, acidified onions and other canned foods sporulate well aerobically on Nutrient Agar with added manganese (5). 

Thermophilic bacteria such as B. stearothermophillus are capable of growth at 55-65°C while an incubation temperature of 30 to 35°C is favorable for culture and sporulation of mesophilic spore formers (5). This property is exploited to grow and therefore differentiate mesophilic and thermophilic spoilage bacteria. As recommended by APHA, in routine diagnosis for spoilage in canned foods, microbiological cultural procedures involve the use of primary recovery media and subculture media to identify spoilage bacteria and study its growth characteristics. Recovery media for aerobes generally include DTA (Dextrose Tryptone Agar) (M092) or DTB (Dextrose Tryptone Bromocresol Broth) (M122).

Use of Cooked Meat Medium (M149) is recommended for recovery of anaerobic organisms. NAMn is widely used as subculture media for aerobes. Nutrient Agar with Manganese supports growth and enhances sporulation by aerobic spore -formers and can be used primarily to differentiate mesophilic from thermophilic Bacillus species. When rod shaped aerobes in pure culture are isolated on DTA (or DTB) media (M092//M122) and sporulation is not evident, the isolates sh ould be subcultured on Nutrient Agar with Manganese, at the temperature of initial isolation.

After incubation for upto 10 days, if spore production has taken place, the spores are heat shocked to destroy all vegetative cells and cultured on Nutrient Agar w/ Manganese at both 30-35°C and 55°C. The temperature at which outgrowth occurs from the spore state indicates whether the isolate is an obligate mesophile (growth at 30 to 35°C), an obligate thermophile (growth at 55°C) or a facultative thermophile (grow th at 30 to 35°C and at 55°C). 

Specification: 

250g