Brucella Broth , 250g

Description: 

Brucella Broth Base is formulated so as to support luxuriant growth of fastidious bacteria like Brucella species (1). Brucella is an intracellular parasite that causes epizootic abortions in animals and septicemic febrile illness or localized infections of bone, tissue or organ systems in humans (2, 3). Brucella species are highly fastidious and therefore require a nutrient rich medium to be able to grow. Also, Brucella species are highly infective and so extreme care should be taken while handling. The basal medium (with add ition of Camplylobacter Supplements) can be also used for the isolation of Campylobacter (4).

Peptic digest of animal tissue, casein enzymic hydrolysate provide organic nitrogen to the organisms. Yeast extract also supply some nitrogenous nutrients but mai nly it serves as a source of Vitamin B complex. Dextrose serves as an energy source. It can be enriched with 5% v/v sterile defibrinated horse blood. For selective isolation of Brucella species, antibiotic mixtures are incorporated into the base (5, 6, 7). When non-selective medium is required, Brucella Broth Base may be employed with the addition of serum only (i.e. without antibiotics).

It is suggested that half the tubes to be incubated in the normal atmosphere, and half in a 10% CO2 enriched atmosphere. Brucella species are highly infectious and so extreme care should be taken while handling. 

Specification: 

250g