Vibrio Cholerae Chromogenic Medium(Chromogenic Vibrio Cholerae Agar) For Separation And Identification of Vibrio Cholera, 1L

Vibrio Cholerae Chromogenic Medium(Chromogenic Vibrio Cholerae Agar)

Usage:
For separation and identification of Vibrio cholera in aquatic products and food poisoning sample.
 
Principle:
Peptone and yeast extract powder to provide a nitrogen source, vitamins, amino acid and a carbon source; sucrose as fermentable sugars; most non-bacteriostatic agents inhibit Vibrio cholerae bacteria, sodium chloride osmotic pressure balance can be maintained; agar as culture medium coagulant; pigment and Vibrio cholerae enzyme has specific reaction, hydrolysis of the substrate, the release of the color groups to produce yellowish green on the plate - blue-green colonies.
 
Formulation (per liter):
Peptone: 15g
Yeast extract powder:5g
Sucrose: 10g
Sodium chloride:5g
Bacteriostatic agent :5g
Agar :13g
Pigment:3.1g
Final pH 9.2 ± 0.1
 
How to use:
1.Suspend 56.1g dissolved in 1000ml of distilled water, according to the proportion of the amplified or reduced. Heated to boiling stirring until completely dissolved, without autoclaving, cooled to about 50 ℃, pour into sterile petri dish.
2.Take 25 g (mL) of test sample with aseptical procedure, adding 225ml of alkaline peptone water , with a rotating blade homogenizer at 8 000 r / min homogenized 1 min, or slap slap type homogenizer 2 min, to prepare a uniform dilution 1:10. If no homogenizer, then samples were placed in a sterile mortar and ground, and then placed in 500 mL of sterile container, add 225 mL of alkaline peptone water and thoroughly shaken.
3.Enrichment: 1:10 dilution above were cultured at 36 ℃ ± 1 ℃ for 6h ~ 8h, if necessary do secondary enrichment.
4.Cross and separation on Vibrio cholerae plate and incubated at 36 ℃ ± 1 ℃ for 18 h ~ 24 h.
5.Further confirmation of Vibrio cholerae hypothetical validation test, such as oxidase, Gram staining.
 
Quality control:
 This product appears light yellow after  pouring into plate, these strains were inoculated after 36 ± 1 ℃ cultured for 18-24 hours growth in the following table:
Bacteria name                 inoculum (cfu / plate)  growth situation   feature
Vibrio cholerae non-VBO 01      30--300              good      green - blue and green
Escherichia coli ATCC 25922      5000              not growth        --
Vibrio vulnificus ATCC27562      5000              not growth        --
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
 
Specifications: Configurable 1L of Medium .