Triple Sugar Iron(TSI) , 250g

Description: 

A composite medium for the differentiation of Ente robacteriaceae according to their ability to ferment lactose, sucrose and glucose, and to produce hydrogen sulphide. 

Not only does this medium perform most of the functions of Kligler Iron Agar but, in addition, its sucrose content permits the recognition and exclusion of sucrose -fermenting species. These organisms may ferment lactose slowly or not at all during the incubation period, but they attack sucrose readily. Some Proteus and other species may give similar reactions to salmonellae and shigellae and it is necessary to distinguish them by their ability to hydrolyse urea. For this reason Triple Sugar Iron Agar should be used in parallel with Urea Broth or Urea Agar. 

This medium was formerly considered to be interchangeable with Kligler medium for the de tection of hydrogen sulphide producing Enterobacteriaceae. It is now thought that Triple Sugar Iron Agar is not suitable for the detection of hydrogen sulphide production by sucrose -fermenting organisms, such as some Citrobacter and Proteus species, in which the sucrose fermentation masks the hydrogen sulphide indicator in the medium^†1. 

Triple Sugar Iron Agar is recommended for the presumptive identification of colonies or sub -cultures from plating media such as Salmonella Shigella Agar (Modified) (CM0533) , Bismuth Sulphite Agar (CM0201), Brilliant Green Agar (CM0263), MacConkey Agar No.3 (CM0115), or Desoxycholate Citrate Agar (Hynes) (CM0227). 

Specification: 

250g