TRNzol Reagent (For purification of RNA from a wide range of samples), Quantity 100ml, DP405-02

TRNzol Reagent is a ready-to-use reagent for the isolation of total RNA from cells and tissues. The reagent is a monophasic solution of phenol and guanidine isothiocyanate. TRNzol Reagent maintains the integrity of the RNA , during sample homogenization or lysis, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. This technique performs well with small quantities of tissue (50-100 mg) and cells (5×106), and large quantities of tissue (>1 g) and cells (>107), of human, animal, plant, or bacterial origin. The simplicity of the TRNzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by TRNzol Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot, and cDNA synthesis.

Storage：

Stored at 2-8℃ and in dark condition for 9 -12 months

Features：

■ TRNzol Reagent is yellow and easy to distinguish between aqueous and organic phase.

■ Purified RNA is free of genomic DNA and other contaminations.

■ High yield RNA for variable kinds of applications.

Required Reagents：

Chloroform, Isopropyl alcohol, 75% Ethanol (in DEPC-treated water), RNase-free ddH2O

Size/Quantity :

100ml