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Description
TIANprep Rapid Mini Plasmid Kit is optimized from traditional alkaline lysis technology, by which high-quality plasmid DNA could be purified within 8 minutes. The new lysis buffer allows the adsorption of DNA onto silica membrane in the presence of high salt. The material that used to make the silica membrane is unique, highly-efficient and highly-specified. This protocol is designed for purification of DNA from 1-4 ml overnight cultures of E. coli.
Features
■ The fastest product to provide high pure plasmid within 6-8 min.
■ Add color indicator to control and remind every process during the experiment ensure a high efficiency of plasmid purification.
■ Could purify more than 85% plasmid DNA from E. coli
Size :
200 preps
Yield
|
Plasmid Type |
Bacterial Cells Volume |
Plasmid Yield |
Plasmid |
|
Low Copy |
1-4 ml |
3-10 μg |
pBR322, pACYC, pSC101, SuperCos, pWE15 |
|
High Copy |
1-4 ml |
6-24 μg |
pTZ, pUC, pBS, pGM-T |