MUG Nutritional Agar (NA-MUG) , 50g

Description: 

MUG Nutritional Agar is the member of faecal coliform group, presence of which in water indicates faecal contamination. These bacteria possess the enzyme b-glucuronidase and are capable of cleaving the fluorogenic substrate 4-Methylumbelliferyl betaD-Glucuronide (MUG) with the release of the corresponding fluorogen, 4-Methylumbelliferone (1). Therefore incorporation of MUG and subsequent fluoroscense is confirmatory for presence of E.coli with no further confirmation required (2).

MUG Nutrient Agar is recommended for detection of E.coli in water and food samples by a fluorogenic method. Presumptive in the samples can be directly inoculated into the medium. Peptic digest of animal tissue, beef extract and yeast extract provide nitrogenous compounds and vitamin B complex. MUG is cleaved by the enzyme beta-glucuronidase of E.coli to release 4-methylumbelliferone which produces visible green-blue fluorescence under long wave UV light (1). Some strains of Salmone and Shigela species also produ ce glucuronidase (3). Refer appropriate references for standard procedures (1). 

Specification: 

50g