MFC Agar , 250g

Description: 

M-FC Agar Base, designed by Geldreich et al (2) is used for the detection and enumeration of faecal coliforms using the membrane filter technique. This medium is based on the property of faecal coliforms to grow at 44 -45°C (1). M-FC Agar Base is recommended by APHA (3) and by various other standards for detection of faecal coliforms (4 -6). APHA recommends the membrane filtration procedure and delayed incubation for faecal coliforms. Proteose peptone, tryptose and yeast extract provide necessary nutrients for the growth of faecal coliforms.

Lactose is the carbon source as well as fermentable carbohydrate in the medium. Bile salts inhibit the growth of contaminating gram-positive microorganisms. Aniline blue is a triphenyl methane dye which suppresses the growth of many gram -positive microorganisms. Aniline blue along with rosolic acid forms the indicator s ystem of the medium. Membrane filters, through which water sample is passed are aseptically placed onto M-FC Agar base plates. If total coliforms are to be estimated, incubation is carried out at 35-37°C whereas if faecal coliform count is to be estimated, incubation is done at 44-45°C. Coliforms will form blue colonies whereas non -coliforms will form gray coloured colonies on M-FC Agar Base. 

Specification: 

250g