EC-MUG Medium , 50g

Description: 

Escherichia coli is a common Gram-negative micro-organism which may be present in food, water and environmental samples. Owing to its importance as an indicator of faecal contamination, continuing efforts have been put into developing rapid and specific me thods for its identification. 

The finding of a highly specific enzyme of Escherichia coli, beta-glucuronidase, has offered a range of opportunities for the improvement of culture media for the identification of this organism. Numerous studies have confirme d a beta-glucuronidase-positive reaction in the middle to upper 90% range of Escherichia coli isolated from a variety of sources¹. The highly sensitive substrate 4-methylumbelliferyl-ß-D-glucuronide (MUG) is cleaved by the enzyme and the hydrolisis product 4-methylumbelliferone shows a visible green/blue fluorescence under long -wave ultra-violet light (366nm). 

EC Broth with MUG contains 4-methyl-ß-D glucuronide which is cleaved by the enzyme glucuronidase (GUD) produced by Escherichia coli resulting in a fluorescent end-product. This medium is recommended by FDA/BAM2 for the confirmation of Escherichia coli from shellfish and meats. For other details regarding the formulation of EC broth with MUG please refer to EC Broth (CM0853). 

NaOH addition generally improves fluorescence by increasing the alkalinity of the solution. An increase in fluorescence can also be achieved by increasing the level of MUG added to the base medium. 

Specification: 

50g