Deoxycholate agar (DC) , 250g

Description: 

An Oxoid modification of Leifson medium1, for the isolation and maximum recovery of intestinal pathogens. It is less selective and inhibiting than Desoxycholate Citrate Agar (Hynes) CM0227 but colonial characteristics are similar on the two media. 

Desoxycholate Citrate Agar provides an opaque background against which one may more easily discern the clearing produced by alkali -producing pathogens. 

The use of a less selective medium for direct sampling of faeces and a more selective medium for post -enrichment sampling, would be advantageous. Similarly, the less inhibitory medium is often preferable when shigellae are being sought as well as salmonellae². 

Directions: 

Suspend 48.5g in 1 litre of distilled water. With frequent agitation bring to the boil over a gauze and flame to dissolve completely. Mix well and pour plates immediately. Dry the agar surface before use. 

THIS MEDIUM IS HEAT SENSITIVE. AVOID EXCESSIVE OR PROLONGED HEATING DURING RECONSTITUTION. DO NOT AUTOCLAVE, OR REMELT. 

Specification: 

250g