Xylose Lysine Desoxycholate Agar (XLD) For Selective Isolation of Gram-negative Bacteria, Final PH 7.4±0.2, 500g/bottle

Xylose Lysine Desoxycholate Agar (XLD)

Usages:
For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
 
Principle:
Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
 
Formulation（per liter）:
Xylose 3.50g
L-Lysine 5.00g
Lactose 7.50g
Sucrose 7.50 g
Sodium Chloride 5.00 g
Yeast Extract 3.00g
Sodium Desoxycholate 2.50g
Sodium Thiosulphate 6.80g
Fe-Ammonium Citrate 0.80g
Phenol Red 0.08g
Agar 13.50
Final PH 7.4±0.2    
 
How to use:
1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes.
2.Dilluted and treated samples.
 
Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows:
strain name              strain code                              growth                feature
Arizona bacteria       CMCC (B) 47001                    good            black colonies
Salmonella typhimurium CMCC (B) 50115          good            black colonies
Streptococcus faecalis    CMCC32223              prohibited                  --        
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 500g/bottle