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Violet Red Bile Agar with Glucose and Lactose for Selective Isolation of Enterobacteriaceae, Final pH 7.4 ± 0.2, 500g/bottle

RM316.00

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Violet Red Bile Agar with Glucose and Lactose
 
Usages:
For selective isolation of Enterobacteriaceae.
 
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
 
Formulation(per litre):
Yeast extract powder 3g
Pancreatic digest of gelatin 7g
Bile salt 1.5g
Lactose 10g
Sodium chloride 5g
D-Glucose Monohydrate 10g
Neutral Red 0.03g
Crystal violet 0.002g
Agar 15g
Final pH 7.4 ± 0.2          
 
How to use:
1.Suspend 51.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, Do not autoclave.
2.Diluted and treated samples.
 
Quality control:
            

Item The name and number of strain Growth Colony Color
1 Escherichia coli ATCC25922 Good Dark red
2 Aerogenes CMCC (B) 45103 Good red
3 Staphylococcus aureus ATCC6538 Suppressed --
 

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 
Specifications: 500g/bottle