Triple Sugar Iron Agar (TSI) For Differentiating Gram-Negative Enteric Bacilli, Final pH: 7.3±02, 500g/bottle

Triple Sugar Iron Agar (TSI)
 
Usages:
For differentiating Gram-negative enteric bacilli.
 
Principle:
Peptone, beef extract powder provide nitrogen, vitamins, minerals; lactose, dextrose, sucrose to fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
 
Formulation (per liter):
Pancreatic Digest of Casein                     10g
Pancreatic Digest of Animal Tissue               10g
Lactose                                     10g
Sucrose                                    10g
Dextrose                                   1g
Ferrous Ammonium Sulfate                    200mg                    
Sodium Chloride                             5g
Sodium Thiosulfate                           200mg
Agar                                      13g
Phenol Red                                 25mg
Final pH                                  7.3±02
 
How to use:
1.Suspend 59.4g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool a slanted position.
2.Diluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 500g/bottle