Tetrathionate Broth Base (Muller-Kauffman Tetrathionate Broth) for Selective Enrichment of Salmonella, Final pH7.0 ± 0.2, 500g/bottle

Tetrathionate Broth Base (Muller-Kauffman Tetrathionate Broth)
 
Usages:
For selective enrichment of Salmonella spp.
 
Principle:
Peptone and beef extract powder provides carbon, nitrogen and vitamins to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; calcium carbonate can neutralize bacterial acid production and absorption of toxic metabolites; bile and brilliant green E. coli and other groups can inhibit Gram-positive bacteria.
 
Formulation (per liter):
Peptone 9g
Beef extract powder 4.5g
Sodium chloride 2.7g
Calcium carbonate 40.5g
Anhydrous sodium thiosulfate 31.9g
Ox bile  5g
Final pH7.0 ± 0.2
 
How to use:
1.Suspend 93.6g in 1L of distilled or deionized water. stirring heated to boiling until completely dissolved, packed in flasks.
2.Place the flask into sterile pot, autoclave at 121 ℃ for 20min, cooled to 30 ℃, adding supplement.
3.Diluted and treated samples.
 
Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows:
strain name              strain code            growth           feature
Salmonella typhi      CMCC (B) 50071           good            turbid broth
Salmonella typhimurium CMCC (B) 50115          good            turbid broth
Escherichia coli          ATCC25922        prohibited              --        
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 500g/bottle
*Supplement : SR0040 Novobiocin