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Terrific Broth Base for Increasing The Yield of Plasmid DNA in E.coli, Final pH7.2 ± 0.2, 500g/bottle

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Terrific Broth Base
 
Usages:
For increasing the yield of plasmid DNA in E.coli.
 
Principle:
Yeast extract provide nitrogen, vitamins and growth factors.
 
Formulation(per liter):
Pancreatic digest of casein  12g
Yeast extract  24g
Dipotassium phosphate 9.4g
Monopotassium phosphate 2.2g
Final pH7.2 ± 0.2
 
 
How to use:
1.Suspend 47.6g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 121 ℃ for 15 minutes.
2.Diluted and treated samples.
 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 
Specifications: 500g/bottle
*Supplement: Glycerol