Mannitol Yolk Polymyxin Agar Base (MYP) for Determination and Isolation of Bacillus Cereus, Final pH7.4 ± 0.2, 500g/bottle

Mannitol Yolk Polymyxin Agar Base (MYP)
     
Usages:
For determination and isolation of Bacillus cereus.
 
Principle:
Peptone and beef extract powder provide nitrogen, vitamins and growth factors; D- mannitol as fermentable sugars; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant; phenol red as a pH indicator; yolk contains lecithin, lecithin to produce Bacillus cereus enzyme precipitation ring around the colonies; and D- mannitol produce acid fermentation Colonies was yellow; polymyxin B inhibit the growth of bacteria.
 
Formulation (per litre):
Peptone 10g
Beef extract powder 1g
D- mannitol 10g
Sodium chloride 10g
Agar 15g
Phenol red 0.025g
Final pH7.4 ± 0.2
 
How to use:
1. Suspend 46g in 1 L of distilled or deionized water ,heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min.
2.Dilluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 500g/bottle
*Supplement: SR0170  Polymyxin