Macconkey Agar for Isolating Lactose-Fermenting Gram-Negative Enteric Bacilli, 500g/bottle

Macconkey Agar
 
Usages:
For isolating lactose-fermenting Gram-negative enteric bacilli.
 
Principle:
Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.
 
Formulation（per litre）:
          

How to use：
1. Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.
 
2. Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42to 44for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30to 35deg.C for 18 to 72 hours.
 
Quality control：
          　　

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.
 
Specifications: 500g/bottle