MRS Broth for Cultivating of Lactobacillus Species, Final pH 5.7 ± 0.2, 500g/bottle 10KG/bag

MRS Broth
     
Usages:
For cultivating of Lactobacillus species.
 
Principle:
Enzymatic digest of casein, beef extract powder, yeast extract powder to provide a nitrogen source, vitamins, growth factors; ammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate to provide the training of various lactic acid bacteria growth factor.
 
Formulation (per liter):
Enzymatic digest of casein 10g
Meat extract powder 10g
Yeast extract powder 4g
Triammonium citrate 2g
Sodium acetate 5g
Magnesium sulfate (MgSO4.7H2O) 0.2g
Manganese sulfate (MnSO4.4H2O) 0.05g
Dipotassium hydrogen phosphate 2g
Glucose 20g
Tween-80 1.08g
Final pH 5.7 ± 0.2
 
How to use:
1. Suspend 54g in 1 L of distilled or deionized water ,heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min.
2.Dilluted and treated samples.
 
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
 
Specifications: 500g/bottle 10KG/bag