Linden Grain Medium ( LG Medium) for Detection of Molds, Yeasts and Total Bacteria, Final pH 6.5 ± 0.2

Linden Grain Medium ( LG Medium)
 
Usages:
For detection of moulds, yeasts and total bacteria, commonly used in aseptic filling empty PET bottle production line for sterility testing.
 
Principle: peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; glucose as carbon source; potassium dihydrogen phosphate as a buffer; ammonium sulfate osmotic equilibrium can be maintained and conducive to the growth of target bacteria.
 
Formulation (per litre):
Glucose :20.0g
Ammonium sulfate:2.0g
Magnesium sulfate:1.0g
Peptone:2.0g
Yeast extract powder: 3.5g
Sodium dihydrogen phosphate: 1.0g
Final pH 6.5 ± 0.2
 
How to use:
1. Weight 29.5g of product, adding 1L of distilled water or deionized water, stirring heated to boiling until complete dissolution, dispensing tube or flask, autoclave at 121 ℃ for 15minutes.
2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination appropriate sample size, amount of medium and positive comparison, culture at 28 ℃ ~ 32 ℃ for 13 ~ 15d.
3. Observe the results.

Quality Control:
Appearance:
Powder: light yellow powder
Prepared broth: yellow, clear and transparent without precipitation.
Biology testing: The following strains were inoculated and cultured at 28 ℃ ~ 32 ℃ for 13 ~ 15d ,growth as below:
Quality control
Strain Name                 Strain Code                   growth characteristics
Pseudomonas aeruginosa        ATCC27853                       turbid broth
Staphylococcus aureus          ATCC6538                        turbid broth
Bacillus cereus                ATCC63303                       turbid broth
Klebsiella pneumonia           ATCC46117                       turbid broth
Hyphae of Aspergillus niger      ATCC16404             flocculent, liquid black spores
Candida albicans               ATCC10231                       turbid broth
 
Storage: Store in a dark, cool and dry place, sealed the product immediately after use.
Storage period of three years
 
Specifications: 021140A 10kg/bag    021140B 1kg/bag