Mannitol Salt Agar For Selective Isolation And Culture of Staphylococcus Aureus, Final PH 7.4±0.2, 500g/bottle

Mannitol Salt Agar

Usages:
For selective isolation and culture of Staphylococcus aureus.
 
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
 
Formulation（per liter）:
Pancreatic digest of casein 5.0g
Pancreatic digest of animal tissue 5.0g
Beef Extract 1.0g
Sodium Chloride 75.0g
Mannitol 10.0g
Phenol Red 0.025g
Agar 15.0g
Final PH 7.4±0.2
 
How to use：
1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
 
Quality control：
          　　

 
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
 
Specifications: 500g/bottle