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Description：
TIANGEN Taq DNA Polymerase is purified from E. coli. expressing a cloned
Thermus aquaticus DNA polymerase gene. The enzyme consists of a single
polypeptide with a molecular weight of approximately 94 kDa. This enzyme
has a 5'→ 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a
3' → 5' exonuclease activity, which results in a 3'-dA overhangs PCR product.
TIANGEN Taq DNA Polymerase can amplify DNA target up to 6 kb. The
elongation velocity is 1~2 kb/min. Taq DNA polymerase is heat-stable and will
synthesize DNA at elevated temperatures from single-stranded templates in
the presence of a primer.
Size/Quantity :
1 ml
Definition of Activity Unit：
One unit is defined as the amount of the enzyme required to catalyze the
incorporation of 10 nmoles of dNT P’s into an acid-insoluble form in 30 minutes at
74°C using hering sperm DNA as substrate.
Quality Control：
The absence of endodeoxyribonucleases, exodeoxyribonucleases and
ribonucleases is confirmed by appropriate quality tests. Functionally tested in PCR.
Storage：
Store at -20°C
