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Introduction：
The pGM-T Vectors are linearized vectors with a single 3′-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases.
The pGM-T Vectors are high-copy-number vectors containing T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of the enzyme β-galactosidase. Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates.
Storage：
Competent cells must be stored at -70℃; other reagents (containing vector and ligation reagents) are stored at -20℃. Subdivision could be taken to avoid repeated freezing and thawing.
Size/Quantity :
20 µl
