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Description:
miRNAs and other noncoding RNAs are polyadenylated by poly(A) polymerase
and subsequently converted into cDNA by reverse transcriptase with oligodT
priming. miRNA s are converted into cDNA by reverse transcriptase using
both oligo-dT and random priming. The cDNA is then used for real-time PCR
quantification of mature miRNA (using a miScript Primer Assay and the miScript
Universal Primer), precursor miRNA (using a miScript Precursor Assay),
noncoding RNA (using a miScript Primer Assay specific for the target and the
miScript Universal Primer), or mRNA (using a QuantiTect Primer Assay).
Storage:
Store at -20°C.