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Description:
Hektoen Enteric Agar was developed by King & Metzger1. The high peptone content offsets the inhibitory effect of bile salts on Shigella species in particular. The additional carbohydrates (sucrose and salicin) give better differentiation than lactose alone and the lower toxicity of the double indicator improves recovery. The increased lactose content helps early recognition of slow lactose-fermenting organisms. The thiosulphate and ferric citrate are present to detect H2S-producing organisms.
Taylor & Schelhaut2 found the medium to be of value in the differentiation of pathogenic organisms and for better growth of shigellae. Hoben et al.3 added novobiocin 15 mg/litre to improve the selectivity of the medium by inhibiting Citrobacter and Proteus species. Hektoen Enteric Agar meets the requirements of the APHA 4.
Specification:
250g