RV Salmonella Enrichment Broth , 250g

Description: 

Rappaport-Vassiliadis (RV) Enrichment Broth is based on the formulation described by van Schothorst and Renaud¹ and is recommended as the selective enrichment medium when isol ating Salmonella species from food and environmental specimens. It can also be used to isolate Salmonella from human faeces without pre -enrichment but the inoculum must be small. The original formulation described by Rappaport et al² was specifically developed to exploit the four characteristics of Salmonella species when compared with other Enterobacteriaceae. 

1. The ability to survive at relatively high osmotic pressures. 

2. To multiply at relatively low pH values. 

3. To be relatively more resistant to mal achite green. 

4. To have relatively less demanding nutritional requirements. 

Rappaport Broth was found to be superior to Selenite Enrichment Broth and Tetrathionate Broth for enrichment of salmonellae with the exception of Salmonella typhi². Vassiliadis et al.³ modified Rappaport Broth by lowering the concentration of malachite green and raising the incubation temperature to 43°C. This modified Rappaport Enrichment Broth is RV or Rappaport-Vassiliadis Medium and has been found to be superior to other salmonella selective enrichment media, especially when small inocula of pre -enrichment broth are used^4,5,6,7,8. 

In an evaluation of different enrichment media for isolation of Salmonella from seawater, Rappaport-Vassiliadis (RV) Broth and the same broth supplemented with novobiocin were the best for de tection and enumeration of salmonellae in samples with low and moderate pollution levels⁹. In another study¹⁰, Rappaport-Vassiliadis (RV) Broth was found to be superior to tetrathionate -brilliant green broth for the detection of salmonellae in artificially contaminated fluid whole milk. 

It is important that the inoculum size used for enrichment culture in RV Broth is sufficiently small not to interfere with its selectivity. Inoculum/broth ratios 1:100 to 1:2000 have been suggested¹². 

Specification: 

250g