Modified VP Medium , 250g

Description: 

Bacillus cereus is widely distributed in nature and can be isolated from a number of foods. However, unless it is able to grow, its presence is not significant to human health. Consumption of foods contaminated with B. cereus cells results in food poisoning. Modified V.P. Broth, formulated as per Smith, Gordon and Clark (1) is used for performing V.P. test. This medium is also recommended by APHA (2) for the confirmation of B. cereus in foods. Modified V.P. Broth has a different composition from the conventional MR-VP Broth used for methyl red and Voges-Proskauer test for differentiating Escherichia and Enterobacter.

Proteose peptone in the medium provides nitrogenous nutrients. Glucose is the fermentable carbohydrate and carbon source in the medium. Acetyl methyl carbinol is produced from glucose by B. cereus . After inoculation and incubation at 35°C for 48 ho urs, the presence of acetyl methyl carbinol is determined by adding 0.2 ml of 40% potassium hydroxide and 0.6 ml of 5% alcoholic # -naphthol solution to 1.0 ml of culture tube. This reaction is hastened by the addition of a few crystals of creatine by which the purple colour development takes place within 15 minutes. 

Specification: 

250g