Lactobacillus Selective Medium (LBS Agar) , 250g

Description: 

Lactobacillus Selection Agar is used for isolation and enumeration of Lactobacilli. Rogosa et al (1, 2) developed LBS Agar as a selective medium for isolation and enumeration of Lactobacilli from oral, faecal specimens (3), food (4) and dairy products (5). Lactobacillus Selection Medium was demonstrated to be more suitable for growth of lactobacilli than Tomato Juice Medium traditionally used to isolate lactobacilli. Lactobacilli Selection Media can be further enriched by addition of tomato juice (6). Casein enzymic hydrolysate, yeast extract and dextrose are the nitrogen and carbon sources. Polysorbate 80 provides fatty acids required for the metabolism of Lactobacilli.

Selectivity of the medium is obtained due to the presence of ammonium citrate and sodium acetate. These inhibit the accompanying microbial and fungal flora and also restrict swarming of colonies (7). A ddition of acetic acid lowers the pH which is inhibitory to many microorganisms but favours the growth of Lactobacilli. on this medium appears as large, white colonies. Growth from Lactobacillus Selection Broth Base (M1166) can be isolated on Lactobacillus Selection Agar Base. Since these media are highly selective, they should not be used for maintenance of lactobacilli.

Specification: 

250g